2.5 Enzymes

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2.5 Enzymes

2.5  Enzymes

Knowledge control of 2.5 Enzymes 

Concept mapping of the 2.5 Enzymes

Essential idea:

2.5.1 Enzymes control the metabolism of the cell.


How do enzymes catalyze reactions? Interactive animation.

Tutorial 6.1 Enzyme Catalysis


Nature of science:


2.5.2 Experimental design—accurate, quantitative measurements in enzyme experiments require replicates to ensure reliability. (3.2)


Enzyme lab




2.5.3 Enzymes have an active site to which specific substrates bind.


How Enzymes Work 

Condensation and hydrolysis with an enzyme

Enzyme Action and the Hydrolysis of Sucrose

Enzyme–substrate specificity


2.5.4  Enzyme catalysis involves molecular motion and the collision of substrates with the active site.


Enzyme specificity

Enzyme animation(basic function, inhibition, allosteric control, feedback inhibition)

Allosteric enzyme

Biochemical Pathway

Biochemical pathways and feedback inhibition

Enzyme animation

Glyceraldehyde -3-Dehydrogenase

Activation energy of enzymes

 Enzyme changing shape

Enzyme catalyzed reaction flashanimation

Enzyme inhibition animation


Enzyme controlled reaction

What is an enzyme?


2.5.5 Temperature, pH and substrate concentration affect the rate of activity of enzymes.


Simulation/Enzymes acting on substrates, under a range of different conditions.


2.5.6  Enzymes can be denatured.


How enzymes work?



2.5.7 Immobilized enzymes are widely used in industry.


Article about enzymes

Worksheet about enzymes




2.5.8 Application: Methods of production of lactose-free milk and its advantages.


Lab ____. A Study Of Enzyme Functıon: Lactose Intolerance




2.5.9 Skill: Design of experiments to test the effect of temperature, pH and substrate concentration on the activity of enzymes. 



2.5.10 Skill: Experimental investigation of a factor affecting enzyme ctivity.(Practical 3)


Enzyme experiment

Amylase (an enzyme) breaking down starch  

Enzyme Experiment Essays and Term Papers




2.5.11 Lactase can be immobilized in alginate beads and experiments can then be carried out in which the lactose in milk is hydrolysed.


2.5.12  Students should be able to sketch graphs to show the expected effects of temperature, pH and substrate concentration on the activity of enzymes.They should be able to explain the patterns or trends apparent in these



Theory of knowledge:


2.5.13 Development of some techniques benefits particular human populations more than others. For example, the development of lactose-free milk available in Europe and North America would have greater benefit in Africa/Asia where lactose intolerance is more prevalent. The development of techniques requires financial investment. Should knowledge be shared when techniques developed in one part of the world are more applicable in another?




2.5.14  Enzymes are extensively used in industry for the production of items from fruit juice to washing powder.


2.5.15 Syllabus and cross-curricular links:



Topic 8 AHL Metabolism, cell respiration and photosynthesis

2.5 Enzymes Past Paper Questions

2.5 Enzymes Presentation

2.5 Enzymes Worksheets

2.5 Enzymes Animations/Videos


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